Engineering Improves Enzymatic Synthesis of L-Tryptophan by Tryptophan Synthase from Escherichia coli
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چکیده
منابع مشابه
Production of a novel tryptophan analog, beta-1-indazole-L-alanine with tryptophan synthase of Escherichia coli.
The tryptophan synthase alpha 2 beta 2 complex from Escherichia coli has been found to catalyze the beta-replacement reaction of L-serine with indazole, an indole analog which has a nitrogen atom at the 2-position (pyrazole ring). The reaction product was isolated and identified as beta-indazolealanine by mass spectrometric, elemental and NMR analyses. Careful assignment of 1H- and 13C-signals ...
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The production of L-tryptophan through chemical synthesis, direct fermentation, bioconversion and enzymatic conversion has been reported. However, the role of transport system for aromatic amino acids in L-tryptophan producing strains has not been fully explored. In this study, the fact was revealed that L-tryptophan production and cell growth were affected by the modification of transport syst...
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Conversion of glucose and ammonium salts into tryptophan by mutants of Escherichia coli was examined as part of a feasibility study on the manufacture of tryptophan. This involved construction, largely by transduction, or a variety of multiple-mutation strains with defined genotypes. By comparing the properties of these strains, we were able to define in biochemical terms several changes that s...
متن کاملRole of D-tryptophan oxidase in D-tryptophan utilization by Escherichia coli.
Mutants of Escherichia coli K-12 that require L-tryptophan (trp) are normally unable to utilize D-tryptophan to fulfill their requirement. However, secondary mutations (dadR) that confer this ability can be isolated. In such strains two distinct enzymes are found to be produced at high levels: D-amino acid oxidase (EC 1.4.3.3) and D-tryptophan oxidase. A convenient assay procedure for D-tryptop...
متن کاملInteractions between tryptophan synthase from Escherichia coli and derivatives of the coenzyme pyridoxal 5'-phosphate.
Coenzyme Derivatives, Pyridoxal 5'-Phosphate, Tryptophan Synthase The interaction of the coenzyme analogues pyridoxal (A), pyridoxine 5'-phosphate (B), pyridoxic acid 5'-phosphate (Q and N-phosphopyridoxyl-L-serine (D) with both the isolated apo ß2 subunit and the native <x2 apo ß2 bienzyme complex of tryptophan synthase from Escherichia coli has been investigated using enzyme kinetics and CD s...
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ژورنال
عنوان ژورنال: Microorganisms
سال: 2020
ISSN: 2076-2607
DOI: 10.3390/microorganisms8040519